A simple, selective, rapid, precise and economical reverse phase high-pressure liquid chromatographic method has been developed for the estimation of 4-(4-(4-(Hydroxydiphenylmethyl)-1-Piperidinyl)-1-Oxobutyl)-α, α-dimethyl benzene acetic acid methyl ester. The method was carried out on a Luna Phenyl Hexyl (250 × 4.6 mm, 5 μm) column, with a mobile phase consisting of Buffer: Acetonitrile pH 2.0 (50:50 v/v) at a flow rate of 1.5 ml/min. The retention time of 4-(4-(4-(Hydroxydiphenylmethyl)-1-Piperidinyl)-1-Oxobutyl)-α, α-dimethyl benzene acetic acid methyl ester was 6.25 min. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation. The proposed method can be used for estimation of 4-(4-(4-(Hydroxydiphenylmethyl)-1-Piperidinyl)-1-Oxobutyl)-α, α-dimethyl benzene acetic acid methyl ester for routine analysis at laboratory.
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